Year: 2021 | Month: December | Volume 11 | Issue 6
An Animal Model to Visualize Differential Expression of Genes Related to Metabolic Process and Immune Reaction between Large White Yorkshire (LWY) and Indigenous Pigs of Punjab
Amanpreet Kaur
Simrinder Singh Sodhi
R.S. Sethi
C.S. Mukhopadhyay
DOI:10.30954/2277-940X.06.2021.2
Abstract:
The pig rearing is renowned area of Indian livestock. It is mainly adopted by tribal folk and economically week classes of country. The pig meat known as pork is highly proteinaceous. The four genes (ACAT2, ACAA2, APOA4 & SOCS2) are evaluated in the current study on the basis of their expression in indigenous pigs of Punjab in comparison to Large White Yorkshire (LWY) where the tissue used is liver. The quality and quantity of RNA transcript is assessed by gel-elctrophoresis and real-time quantitative PCR. All genes show significant difference (P<0.05) in both breeds except ACAA2 (P>0.05). The expression of ACAT2 is higher in indigenous breed whereas other three genes downregulates in indigenous breed of pigs. The mRNA expression of APOA4 is negligible i.e. only 1% expression of LWY. On the behalf of this data, we can say that indigenous pigs have good cholesterol metabolism as ACAT2 is higher in indigenous pigs but are more prone to cardiac diseases, gastric ulcers and other chronic diseases due to low expression of APOA4 and SOCS2. This study can be helpful to find the genetic differences between LWY and indigenous pigs of Punjab.
Highlights
- We studied comparative expression profiling of genes in liver of indigenous and Large White Yorkshire breeds of pigs
- The present study will help in genetic profiling of genes responsible for regulating metabolism and immune response
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